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Figure 3.

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Salmonella Choleraesuis ∆crp was less invasive in porcine ligated ileal loops and in macrophages. The degree of intestinal invasion by wild type Salmonella Choleraesuis (WT) and CP327 was determined at 3 h after inoculation of the loops (A). To recover bacteria, portions of the ileal wall with Peyer patches (PP) and associated mesenteric lymph nodes (LN) were harvested, homogenized, and plated onto BG agar. The results are from at least triplicate determinations. For in vitro invasion (B) and intracellular survival assays (C), porcine alveolar macrophages were co-cultured with WT and CP327 of Salmonella or E. coli (K12) strains at an MOI of ~ 20 for 2 h and intracellular survived bacteria were assessed. Relative invasion was determined at 4 hpi while intracellular survival rates were at 8 and 16 hpi. The results are representatives of three independent experiments of at least triplicate determinations. Error bars are SEM. *p < 0.05 and ***p < 0.001 compared to WT; n.s.: no significant difference from each other (ANOVA).

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