Open Access
Table I.
BALF-glycoproteins that have been identified by mass spectrometry analysis. The identity of the proteins was determined either by MALDI-TOF MS or by ESI Q-TOF MS/MS fragment mass fingerprint (italic) in combination with a database search (MASCOT, BLAST).
| Gel spot | Protein name | Accession number | Theor. pI | Theor. MW (kDa) | Peptide matches | Sequence coverage (%) |
|---|---|---|---|---|---|---|
| 1 A/B/C/D | alpha-1-acid glycoprotein | Q_29014 | 5.83 | 20 899 | 2/2/2/2 | 12/12/12/12 |
| 2 A/B/C/D/E/F/G | alpha-1-antichymotrypsin 3 | CAC06756 | 5.77 | 22 883 | 8/16/19/14/10/13/3 | 12/42/44/34/33/29/17 |
| 3 B | alpha-1B glycoprotein (canis familiaris) | XP_541346 | 5.54 | 55 580 | 1 | 2 |
| 4 A/B | alpha-2-HS-glycoprotein / fetuin A | P29700 | 5.74 | 38 400 | 1/2 | 6/6 |
| 5 A | complement component 3 | NP_999174 | 6.09 | 188 229 | 13 | 11 |
| 6 A | ficolin | NP_999033 | 5.63 | 35 173 | 11 | 33 |
| 7 A | haptoglobin precursor | Q8SPS7 | 6.93 | 38 457 | 1 | 4 |
| 8 A/B/C/D/E/F/G | haptoglobin | NP_999165 | 6.93 | 38 457 | 10/9/10/11/10/10/8 | 34/23/35/33/34/35/27 |
| 9 D/E | hemoglobin, alpha-subunit | P_01965 | 8.76 | 15 087 | 8/8 | 64/64 |
| 10 B | hemoglobin | 1QPW_D | 7.21 | 16 025 | 2 | 15 |
| 11 A/B/C/D/E | hemopexin / hyaluronidase | NP_999118 | 6.59 | 52 071 | 2/15/16/16/10 | 8/29/26/32/23 |
| 12 A | Ig heavy chain variable region | CAJ45440 | 4.85 | 13 573 | 8 | 65 |
| 13 A | Ig lambda chain C region | P01846 | 6.75 | 11 168 | 4 | 65 |
| 14 A/B/C/D/E/F/G/H/I/J/K | inter-alpha trypsin inhibitor family heavy chain related protein | NP_001001537 | 6.42 | 102 254 | 15/16/18/18/19/25/24/31/24/21/19 | 15/20/19/19/23/28/26/34/30/24/26 |
| 15 D/E/F/G/H | properdin, B-factor | NP_001095294 | 7.45 | 87 179 | 18/16/18/11/11 | 31/21/24/18/15 |
| 16 A | pulmonary surfactant-associated protein D | NP_999275 | 8.57 | 37 961 | 1 | 5 |
| 17 B/C/D/E/F/G | transferrin | CAA30943 | 7.08 | 76 901 | 12/16/7/20/18/15 | 25/22/11/30/27/19 |