Breeding line-specific BALF glycoprotein regulation comparing day −7 and day 4/20 post infection. For three pigs of each breeding line, glycoproteins obtained from BALF on day −7, 4 and 20 were enriched by lectin affinity chromatography and separated by 2-D PAGE. Gels were performed for three biological replicates, and silver-stained protein spots were quantified by ImageJ software. Protein expression on day 4 or on day 20 was compared to day −7. If the quotient was below 1, the negative inverse value is given. Diamond symbols indicate relative expression differences of individual proteins on day 4 or 20 in comparison to day −7. Mean values are depicted by black bars. Significant differences are indicated by asterisks (* ≤ 0.05, ** ≤ 0.01, *** ≤ 0.001). AGP: alpha-1-acid glycoprotein, AHSG: fetuin A, Hp: haptoglobin, Hb: hemoglobin, HPX: hyaluronidase/hemopexin, SERPINA3: alpha-1-antichymotrypsin 3, SP-D: pulmonary surfactant-associated protein D. For German Landrace (Hb, day 20), Piétrain, (AGP day 20, Hb and SP-D days 4 and 20), and Hampshire (Hb day 20) the respective proteins were below detection level in individual pigs and, therefore, no expression ratios could be obtained in these cases.