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Figure 1.

Silver-stained 2-D-PAGE from a representative Piétrain pig prior to infection (A) and on day 4 post infection (B) with A. pleuropneumoniae. Purification of glycoproteins was carried out by concanavalin A lectin chromatography. Identities of differential expressed proteins were detected by mass spectrometry (MALDI-TOF MS, ESI Q-TOF MS/MS) and data base analysis (MASCOT, BLAST).