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Table II.
Details of primers used in sequencing sheep genes and for RT-PCR.
| Gene | Primer sequence | Product size (BP) | |
|---|---|---|---|
| sITLN ext. GSP 2 | Fw | GGT GGC GGC TGG ACC CTG GTG | 309 |
| sITLN ext. GSP 1 | Rv | GCT CCG GAA GAA GCC CGT GTT GG | |
| sITLN int. GSP 2 | Fw | CAC GGT GGG CGA TCG CTG GTC | 176 |
| sITLN int. GSP 1 | Rv | GCA CGT GCC AGA TGC CCA GGT C | |
| sITLN F1 | Fw | GCT CTG AGA CTG CTC CTG GT | 420 |
| sITLN ext. GSP 1 | Rv | GCT CCG GAA GAA GCC CGT GTT GG | |
| sITLN F1 | Fw | GCT CTG AGA CTG CTC CTG GT | 1 182 |
| sITLN R1 | Rv | TTC TCC TTT GCT TGT TGG AGA | |
| sITLN F3 | Fw | GCA TCT GTG AGG AAG GAA GG | 1 112 |
| sITLN R3 | Rv | TTC CAT TTC TGA GGG TCT GG | |
| sITLN F1 | Fw | GCT CTG AGA CTG CTC CTG GT | 1 125 |
| sITLN R9-3 | Rv | TGG GTT CCA CAT CTC ATA GA | |
| sITLN F10 | Fw | GCGAGAATTTGTTGCAGGAT | 417 |
| sITLN R10 | Rv | CCAGGGGTCAGTCAGTTTGT | |
| sITLN F7 | Fw | AGGGGACAGGAGTCAGGTTT | 1 000 |
| sITLN R10 | Rv | CCAGGGGTCAGTCAGTTTGT | |
| sATPase F | Fw | GCT GAC TTG GTC ATC TGC | 167 |
| sATPase R | Rv | CAG GTA GGT TTG AGG GGA TAC | |
| sITLN F10 | Fw | GCGAGAATTTGTTGCAGGAT | 287 |
| sITLN R9-1 | Rv | TGG CTT TCT GTC TCA CAC CA | |
| sITLN F10 | Fw | GCG AGA ATT TGT TGC AGG AT | 286 |
| sITLN R9-2 | Rv | TGG GTT CAC ATC TCA TAG A | |
| sITLN F10 | Fw | GCG AGA ATT TGT TGC AGG AT | 287 |
| sITLN R9-3 | Rv | TGG GTT CCA CAT CTC ATA GA | |
| BD Smart 11 A oligonucleotide | AAGCAGTGGTATCAACGCAGAGTACGCGGG | ||
| 3′-Race CDS primer A | AAGCAGTGGTATCAACGCAGAGTAC(T)30 | ||
| 5′-Race CDS primer | (T)25VN | ||
| 10 × universal primer A long | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | ||
| 10 × universal primer A short | CTAATACGACTCACTATAGGGC | ||
Forward (Fw), reverse (Rv), intelectin (ITLN), sheep (s), base pair (BP), external (ext.) and internal (int.).