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Table II.
Real-time PCR primers and probes.
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | Taqman probe (5′-3′) | Amplicon size (bp) |
|---|---|---|---|---|
| Pmp1B | GCTACAGAGGATCGGGATTAAGTG | TCCGCTTGCATCTTGTGTTTG | TTCAGATTCTGCATCAAAGAATTTCACGCA | 77 |
| pmp2A | TTTAGCGAAAACTCCGCAGAA | AATGGGCTGGGAAGCTATAAGG | CGGTGGAGCTATAACCGCGAGCAG | 74 |
| pmp3E | GGTGGCCAAGGCTTGGT | CGGAATCCTGGCACACCTA | ACGGCTATCCACCAAGCGAATCGC | 61 |
| pmp4E | AGGATACATTCCCAACCCCATA | TAGGCGGCTTGCCAAAGA | ATCGTGGAGATCTTGTGGCTAATG | 65 |
| pmp5E | TCCGTCACTTTGGGAATTAGAAG | TCCGTCACTTTGGGAATTAGAAG | TGCGTATAAACCTAACGCCGTCCGAA | 74 |
| pmp6H | TGCCTCATGCAATATCGAAAAT | GCCGCAAGCAGCAAAGTT | ACTCTGCGGCTATCTCGTTTTCT | 66 |
| pmp7G | TGCTGCTGAGGTAAGTAGCGTTA | GCTATTCGCTTGATTGGTTACAGA | CGTCGACTGCAGCTACTGCGGC | 72 |
| pmp10G | GCCAACCACTCCTTAATATTTGAAGA | TGGTTTTGCCATCCGTATTTG | ATCGTCTCTACAGCTCAAGGAGCTGCTATCAG | 81 |
| pmp11G | TGCCTTATCCACTGCTACCAAA | GGAGCCGTTGAATCCAGTTG | TTCTTGCAGATGCCGACTCTGTCAACC | 72 |
| pmp13G | TCTTTAATCCTTAAAGATGGTGTCACTCT | CGTAGTCCCTAGGTCCATGACAA | AAATCCTTCACGCAAACAGAGGGCG | 89 |
| pmp14G | GGAGCATTCAGTCCGCAATC | TCTACAATAGAAATATCACTCTCGACGTT | ACAAGCACTGCGGGAGGAACGATTT | 76 |
| pmp15G | CCCTTATCTTTCACCGCATCA | TCGGTGGTGTTTTGGTTTCA | CGGATGTTGCCAATGAGCATGGC | 75 |
| pmp16G | CTCAATGCTAAAGAAGGTTTTGGTATT | GAGAAGACGATCTTTCCTGTATAAGTAGTG | TCTTTTATGACCCTATCGCTAACACAGGAGGATC | 124 |
| pmp17G | GGCACAGGGAAATTTACAAAGC | CTCCCCCAGTAATAGGGTCATAGA | ACGTGCTAAAGACGGCTTCGGAATTTTC | 75 |
| pmp18D | TCCACTGGGATGATCACCAATA | GCATAGAAAGCGTATCGAGAATCAC | TGATAAATTATATGGTTATGTACCAGCTTC | 81 |
| omp1 | GCGGCATTCAACCTCGTT | CCTTGAGTGATGCCTACATTGG | TGTTAAAGGATCCTCCATAGCAGCTGATCAG | 85 |
| 16S | AATCTTTCGCAATGGACGAAA | ACAACCCTAGAGCCTTCATCACA | TCTGACGAAGCAACGCCGCG | 66 |