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Table I.
Genes, GenBank accession numbers of the sequences used for primer design and origin species. Primers (F: forward and R: reverse) and TaqMan probes (P) used for gene amplification. Length of the amplicon and GenBank accession numbers for the new ovine cDNA sequences. Real-time RT-PCR conditions: annealing temperature, primer and probe concentrations, correlation coefficient (r2) and slope of the standard curve.
| Genes | GenBank | Species | Primers and Probes | bp | New ovine sequences | PCR conditions |
|||
|---|---|---|---|---|---|---|---|---|---|
| Ta | (nM) | r2 | Slope | ||||||
| AIF | AF529274 | Ovine | F: TGGAGGCACAGCTGCTTTT | 94 | 59 | 900 | 0.991 | −3.44 | |
| R: CGGCAGCTCAGGATCTTCA | 900 | ||||||||
| APAF1 | XM_617966 | Bovine | F: GCCAAGCAGGAGGTCGATAA | 117 | EF447257 | 60 | 900 | 0.995 | −3.24 |
| R: GCAAGCATGGTAAACAGCATCT | 900 | ||||||||
| BAD | NM_001035459 | Bovine | F: CAGAGTTTGAGCCGAGTGAG | 110 | AY547260 | 60 | 300 | 0.982 | −3.58 |
| R: GGCTGTTAGCCAGTGCTTG | 300 | ||||||||
| BAK | AF164518 | Ovine | F: CCCAGAGCCTACCAGCACC | 164 | 60 | 300 | 0.997 | −3.3 | |
| R: ACAGGCTGGACGCGATCTT | 300 | ||||||||
| BCL2 | AY423861 | Ovine | F: TGGTGGAGGAGCTCTTCAGG | 65 | 60 | 300 | 0.995 | −3.39 | |
| R: TCCGAACTCAAAGAAGGCCA | 300 | ||||||||
| P: ATGCGCCCCCAGTTCACCCC | 150 | ||||||||
| BCL-XL | AF164517 | Ovine | F: CAGGCGATGAGTTTGAACTG | 121 | 60 | 300 | 0.996 | −3.32 | |
| R: TCCCGGAAGAGTTCATTCAC | 300 | ||||||||
| P: CGACCTGACGTCCCAGCTCCACA | 150 | ||||||||
| BCL-XS | AY568555 | Ovine | F: TCAGAGCTTTGAACAGGACACG | 98 | 60 | 300 | 0.993 | −3.42 | |
| R: TCAGGAACCAGCGGTTGAAG | 300 | ||||||||
| FAS | AB011671 | Ovine | F: CTGGAGCAAGTTCCTGCCAA | 105 | 60 | 300 | 0.996 | −3.14 | |
| R: CTCCGTCGCGTTTGC | 900 | ||||||||
| FASL | XM_584322 | Bovine | F: GAAGAGGAGGGACCACAACACA | 147 | EF458007 | 61 | 900 | 0.990 | −3.32 |
| R: CTTTGGCTGGTGGACTCTCTGA | 900 | ||||||||
| P: TGGTGGCCCTGGTTGGATTGGG | 200 | ||||||||
| MCL1 | AF144097 | Ovine | F: GGCTTTCCAAGGCATGCTT | 112 | 60 | 900 | 0.997 | −3.32 | |
| R: GAGTCACAATCCTGCCCCAG | 900 | ||||||||
| P: CGAGTGATGGTTCATGTTTTCAGTGACG | 150 | ||||||||
| PARP1 | D90073 | Bovine | F: AGTGAAGGCCACGATTGAGAA | 147 | EF458008 | 59 | 900 | 0.991 | −3.32 |
| R: TCAGACACGACACGGATGTTG | 900 | ||||||||
| TGFB1 | X76916 | Ovine | F: GCACGTGGAGCTGTACCAGAA | 125 | 60 | 900 | 0.998 | −3.32 | |
| R: GCCACTGCCGCACAACTC | 900 | ||||||||
| TNFA | X56756 | Ovine | F: CCCTTCCACCCCCTTGTTC | 277 | 60 | 900 | 0.979 | −3.32 | |
| R: GGCTCTTGATGGCAGAGAGGAT | 900 | ||||||||
| TNFR1 | U90937 | Bovine | F: CTCAGGACCCAGGCACTACAG | 134 | EF447258 | 59 | 900 | 0.998 | −3.25 |
| R: CCCGCAAATGATGGAGTAGAG | 900 | ||||||||
| TNFR2 | NM_001040490 | Bovine | F: GATGGGTCCTGTCTTGGTGTGT | 144 | EU542424 | 60 | 300 | 0.993 | −3.32 |
| R: CAAAACAACAAGGGCTCCAGAA | 300 | ||||||||