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Figure 2.


1D-substrate analysis of 2 μg cystatin-binding fraction. The fractions were incubated before and after electrophoresis with only DTT (lane 1), DTT and recombinant cystatin (lane 2), DTT and AEBSF (lane 3), DTT and E64 (lane 4), DTT, EDTA, AEBSF and Pepstatin (lane 5) and unrelated recombinant (Cp-ASP-1a) (lane 6). Molecular markers are indicated in the middle (in kDa).

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