Free Access
Table I.
Primers used in this study.
| Primer | Sequence 5′-3′ | |
|---|---|---|
| Bac17-F | GGATCCATGGAGGCGCATATAGAAAGCGAA | Underlined, BamHI site |
| Bac17-R | GCGGCCGCTCACATATACACCTCTGAAACGTA | Underlined, NotI site |
| pF-Forw | GATATCCGCCACCATGTTTGCAGTGAGCGC | Underlined, EcoRV site |
| pF-Rev | AGATCTTACACAGCTGTCTGAGACATCG | Underlined, BglII site |
| 2×-HA-Fw | GAATTCTATCCGTATGATGTTCCTGATTATGCTAGCCTCTATCCGTATGATGTTCCTGATTATGCTAGCCTCGTGTAGGCTGGAGCTGCTTC | Bold, EcoRI site; underlined, 2×HA tag sequence |
| 2×-HA-Rev | GAATTCCATATGAATATCCTCCTTAG | Bold, EcoRI |
| UL17-Fw | AAGATGCAACAGGTGCTACGACCTTACGTTTCAGAGGTGTATATGTATCCGTATGATGTTCCTGATTAT | Underlined, sequences annealing to template plasmid pSU311-2×HA |
| UL17-Rev | TCTTCAGTCTCTGCGTAGTCATAATTCAATACCTGTGTTGAGGATTGGCATATGAATATCCTCCTTAG | |
| UL49-Fw | CGTCGCCATCATTCATCCGCCCGCGGATATCGTAGCAGTGATAGCGAATATCCGTATGATGTTCCTGATTAT | |
| UL49-Rev | TAGATAAGATATATATGTACTAGTTTTTAATTCGGATGTCTATAAAAGCATATGAATATCCTCCTTAG | |
| UL48-Fw | AATTCAATTTCCCCTGGTGATCCTGTTGCCACTACCATCAGTACTTTATATCCGTATGATGTTCCTGATTAT | |
| UL48-Rev | CGAATACGACAAATTCGTTTATTAAACGTCACATTTACGTATAATATCCATATGAATATCCTCCTTAG | |
| PX1 | GCGGCAGCGTGAGGGGATCTTGAAG | Specific of KanR gene |
| PX2 | CCCCAGCTTCAAAAGCGCTCTGA | Specific of KanR gene |
| T497 | GCATCTAGCACTTGGAGA | Specific of UL49 gene |
| T500 | CCCTGCCCATGCCTCCCA | Specific of UL48 gene |
| DelTag17 | CAGGTGGCTGTGTACGTTC | Specific of UL17 gene |
| UL17delFw | GGTATCTTCAGTCTCTGCGTAGTCATAATTCAATACCTGTGTTGAGGACATATGAATATCCTCCTTAG | Deletion of UL17 |
| UL17delRev | GACCTTAGTCACAACATTGGTGCACGTAGGCAACACGACGGATAACAGCGGTGTAGGCTGGAGCTGCTTCG | |
| UL17delRev2 | GAAACTATATATTCATTATCTAACTTTGATCGGGGGGCGTAGCGACATCTGTTAACACATAGGATGACGACGATAAGTAGGG | Mutation of the 2d start codon of UL17. Bold, complementary sequences; grey box, mutation; underlined, sequences annealing to template plasmid pEPkan-S |
| UL17delFw2 | CATTCATCAGGTATTATTATATGTGTTAACAGATGTCGCTACGCCCCCCGATCAAAGTTACAACCAATTAACCAATTCTGATTAG | |
| UL17ResA | CTATGCACGGTGTCGTCGGT | |
| UL17ResB | TCCTGCATAAACGAATCCGG | |
| UL17seqA | CCCGTAGCATTCTGTCGTATTTGAACG | |
| UL17RFP1 | gtatcttcagtctctgcgtagtcataattcaatacctgtgttgaggattggTCACAAGGCGCCGGTGGAGTGG | Uppercase, sequences annealing to template plasmid pEPmRFPin |
| UL17RFP2 | accaacaagatgcaacaggtgctacgaccttacgtttcagaggtgtatatgATGGCCTCCTCCGAGGACGTCATC | |
| B17F | cccgGGATCCatggaggcgcatatagaaag | Underlined, BamHI site |
| Xh17r | agatCTCGAGctacacgaggctagcataatc | Underlined, XhoI site |