Expression and subcellular location of MDV-1 pUL17. CESC were infected with vRB-1Btag17 (A) or vRB-1B (B) and stained for pUL17 (anti-HA tag-Red) and either VP5 (A) or VP22 (B) (green). The localisation of pUL17 is unaffected by the size of the tag sequence (C): CESC were infected with vRB-1B-UL17mRFP (left) or vBac20UL17mRFP (right). The mRFP tagged protein displays the red signal in nuclei where the presence of VP5 is also detected (green).