Effect of inhibiting bacterial protein synthesis on phagocytosis. FITC-labelled strains of H. parasuis were incubated with PAM in the presence of chloramphenicol (Cm; dashed bars) or without the inhibitor (plain bars). After 1 h, extracellular bacteria were detected with a H. parasuis-specific antiserum coupled with a phycoerythrin-secondary antibody and macrophages were analysed by flow cytometry. Results are presented as the percentage of macrophages with total bacteria (black bars), internalized bacteria (dark grey bars) and surface associated bacteria (light grey bars).