Open Access
Figure 5.

Effect of cytD on the phagocytosis of H. parasuis strains. FITC-labelled strains of H. parasuis were incubated with PAM with (dashed bars) or without (plain bars) cytD. After 1 h, extracellular bacteria were detected with a H. parasuis-specific antiserum coupled with a phycoerythrin-secondary antibody and macrophages were analysed by flow cytometry. Results are presented as the percentage of macrophages with internalized bacteria (black bars) and surface associated bacteria (grey bars).