Time course of Hp protein released into RPMI 1640 medium by somatic cells from groups A and D ex vivo. Freshly isolated somatic cells from milk samples of groups A and D (n = 3 of each group) at 4 °C were immediately suspended in RPMI 1640 and kept at 37 °C over time. Supernatant containing Hp was determined using an ELISA. Protease inhibitor (PMSF) was also added in parallel to each group in an attempt to minimize the Hp degradation. Each bar represents the mean ± SEM.