Early immune response following Salmonella enterica subspecies enterica serovar Typhimurium infection in porcine jejunal gut loops | Veterinary Research, a journal on Animal Infection

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Table II.

Statistical comparisons between mRNA levels of expression. Levels of expression are shown in the second column (High: Amplification around 17–24 cycle thresholds (Ct), very low more than 33 Ct). When the data were paired and normally distributed, group means were compared using Student’s Paired t-test. Paired, non-normally distributed data were analysed using the Wilcoxon Signed Rank Test (Exact).

Messenger RNA	Level of expression	P value
		cPP vs. iPP	cGW vs. iGW	cPP vs. cGW
APRIL/TNFSF13	High	0.096 >	0.127	0.069 <
BAFF/TNFSF13B	High	0.006 ** <	0.058 <	0.009 ** >
CCL20/MIP3 alpha	High	0.368	0.288	0.510
CCL25/TECK	High	0.008 ** >	0.009 ** >	0.121
CCL28/MEC	Moderate	0.381	0.114	0.027 * <
CCR9	High	0.004 ** >	0.559	0.227
CCR10	Moderate	0.433	0.213	0.148
CCRL1/CCR11	High	0.059 >	0.318	0.573
CD40L	Moderate	0.128	0.382	0.004 ** >
CX3CL1/Fractalkine	Moderate	0.1727	0.007 ** <	0.083 >
CX3CR1	Low	0.671	0.236	0.103
CXCL2/GRO beta	Moderate	0.117	0.013 * <	0.637
CXCL10/IP-10	High	0.002 ** <	0.010 ** <	0.565
FOXP3	Low	0.609	0.305	0.001 ** >
GATA3	Low	0.220	0.399	0.048 * >
GM-CSF/CSF-2	Low	0.452	0.156	0.227
IL2/TCGF	Low	0.201	0.608	0.038 * >
IL1 beta/LAF	Very low	na	na	na
IL4/BCGF	Very low	0.616	0.061 <	0.383
IL5/EDF	Moderate	0.086 >	0.375	0.639
IL6/IFN beta 2	Moderate	0.013 * <	0.039 * <	0.592
IL8/CXCL-8	High	0.149	0.001 ** <	0.843
IL10/B-TCGF	Moderate	0.118	0.067 * <	0.806
IL12 p35	Moderate	0.009 ** <	0.001 ** <	0.003 ** >
IL12 p40	Moderate	0.003 ** <	0.027 * <	0.018 * >
IL13	Very low	0.151	0.091 <	0.415
IL15/IL-T	Moderate	0.263	0.142	0.813
IL17a/CTLA8	Low	0.541	0.062 <	0.239
IL18/IGIF	High	0.150	0.869	0.187
IL21	Low	0.319	0.553	0.238
IL22/IL-TIF	Moderate	0.100	0.761	0.684
IL23 p19	Moderate	0.325	0.922	0.036 * <
IL27 p28	Moderate	0.019 * <	0.105	0.239
IL33	Moderate	0.022 * <	0.703	0.322
IFN gamma	Moderate	0.010 * <	0.009 ** <	0.237
iNOS	Moderate	0.009 ** <	0.015 * <	0.831
LTA/TNF beta	Low	0.972	0.715	0.141
MAdCAM-1	High	0.244	0.148	0.410
NK-lysin	High	0.164	0.354	0.181
NOD2	Moderate	0.013 * <	0.004 ** <	0.102
PBD-1	Moderate	0.213	0.051 >	0.065 >
PBD-2	Moderate	0.017 * <	0.280	0.025 * <
PMAP-37/cathelicidin	Very low	0.013 < <	0.289	0.134
PR-39	Very low	0.106	0.089 >	0.027 * >
RORC/ROR gamma	Moderate	0.340	0.201	0.035 * <
Secretory component	High	0.026 * >	0.037 * >	0.122
SMAD2	High	0.648	0.613	0.052 >
SMAD3	Moderate	0.070 >	0.849	0.644
STAT3	High	0.606	0.224	0.856
STAT4	Moderate	0.363	0.015 * <	0.018 * >
STAT5	Moderate	0.828	0.846	0.629
STAT6	Moderate	0.828	0.691	0.155
T-Bet	Low	0.710	0.207	0.127
TGF beta	Moderate	0.253	0.002 ** <	0.005 ** >
TLR2	Moderate	0.002 ** <	0.044 * <	0.797
TLR4	Moderate	0.001 ** <	0.020 * <	0.021 * >
TLR5	Moderate	0.020 * >	0.001 ** >	0.514
TNF alpha/TNFSF2	Moderate	0.007 ** <	0.002 ** <	0.004 ** >

cPP: Control Peyer’s Patch (PP); iPP: Infected PP; cGW: Control Gut Wall (GW); iGW: Infected GW. *P < 0.050, **P < 0.010. na: Not available, expression too low. Higher (>) or lower (<) mRNA expression in the control than in the infected tissues.