Open Access
Table I.
Primer sequences, annealing temperatures of primer sets, expected PCR fragment sizes and accession numbers.
| Primer name | Primer sequence | Annealing temperature (°C) | PCR product (bp) | Accession number |
|---|---|---|---|---|
| APRIL/TNFSF13 | S: TGCTCACCCGTAAACAGAAG | 60 | 172 | EST BP170456 |
| AS: TAAACTCCAGCATCCCAGAC | ||||
| BAFF/TNFSF13B | S: GAGAGCAGCTCCATTCAAAG | 60 | 103 | NM_001097498 |
| AS: GCATGCCACTGTCTGCAATC | ||||
| CCL20/MIP3 alpha | S: GCTCCTGGCTGCTTTGATGTC | 66 | 146 | NM_001024589 |
| AS: CATTGGCGAGCTGCTGTGTG | ||||
| CCL25/TECK | S: GCCTACCACAGCCACATTAAG | 64 | 136 | NM_001025214 |
| AS: GCTTCCCGCACACCATCTT | ||||
| CCL28/MEC | S: GCTGCTGCACTGAGGTTTC | 62 | 144 | NM_001024695 |
| AS: TGAGGGCTGACACAGATTC | ||||
| CCR9 | A: TACGGCTATGACGCCACACC | 69 | 143 | NM_001001624 |
| AS: ACGGCACCCACGATGAACAC | ||||
| CCR10 | A: GCCCGCAGAGCAGGTTTCC | 66 | 136 | NM_001044563 |
| AS: CAAAGAGACACTGGGTTGGAA | ||||
| CCRL1GSP | S: CTGCCTGCCTTCTTCACAGTAGCTTTCATC | 72 | na | EST BW955277 |
| AS: CCACCCATGAACTGCATTAACTGCCCAGAAG | ||||
| CCRL1nGSP | A: CCTTCTATTCACTCTGCCCTTCTGG | 72 | na | EST BW955277 |
| SS: CCCGCCCTTGTCTTACATCCAGGAG | ||||
| CCRL1/CCX-CKR | A: ACAGATACTGGGCAGTAACG | 61 | 147 | NM_001097430 |
| AS: CACACCTCGCTTTGTGATTG | ||||
| CD40L | A: TACGCCCAAGTCACCTTCTG | 62 | 172 | AF248545 |
| AS: AGACTCCGCCCAAGTGAATG | ||||
| CX3CL1/Fractalkine | S: GCAGCTCCTAGTCCATTAC | 58 | 167 | EST CK464144 |
| AS: CACCATTCTGACCCAGAAG | ||||
| CX3CR1 | A: ACCTTGCCCTTCTGGACTC | 60 | 182 | EST BX919199 |
| AS: ACGGTCCGGTTGTTCATGG | ||||
| CXCL2/GRO beta | S: TGCTGCTCCTGCTTCTAGTG | 60 | 171 | NM_001001861 |
| AS: TGGCTATGACTTCCGTTTGG | ||||
| CXCL10/IP-10 | S: CCCACATGTTGAGATCATTGC | 60 | 168 | NM_001008691 |
| AS: CATCCTTATCAGTAGTGCCG | ||||
| FOXP3 | A: GGTGCAGTCTCTGGAACAAC | 65 | 148 | AY669812 |
| AS: GGTGCCAGTGGCTACAATAC | ||||
| GATA3 | A: CCCGTCCTACTACGGAAAC | 60 | 193 | EST BW971285 |
| AS: GTGGTGGATGGACGTCTTG | ||||
| GM-CSF/CSF-2 | S: GAAACCGTAGACGTCGTCTG | 62 | 150 | DQ108393 |
| AS: GTGCTGCTCATAGTGCTTGG | ||||
| HPRT-1 | S: GGACTTGAATCATGTTTGTG | 60 | 91 | DQ815175 |
| AS: CAGATGTTTCCAAACTCAAC | ||||
| IL1 beta/LAF | S: AGAAGAGCCCATCGTCCTTG | 62 | 139 | NM_001005149 |
| AS: GAGAGCCTTCAGCTCATGTG | ||||
| IL2/TCGF | S: GCCATTGCTGCTGGATTTAC | 63 | 159 | NM_213861 |
| AS: CCCTCCAGAGCTTTGAGTTC | ||||
| IL4/BCGF | S: CAACCCTGGTCTGCTTACTG | 65 | 173 | NM_214123 |
| AS: CTTCTCCGTCGTGTTCTCTG | ||||
| IL5/EDF | S: TGGAGCTGCCTACGTTAGTG | 64 | 105 | NM_214205 |
| AS: TCGCCTATCAGCAGAGTTCG | ||||
| IL6/IFN beta 2 | S: ATCAGGAGACCTGCTTGATG | 62 | 177 | NM_214399 |
| AS: TGGTGGCTTTGTCTGGATTC | ||||
| IL8/CXCL-8 | S: TCCTGCTTTCTGCAGCTCTC | 62 | 100 | NM_213867 |
| AS: GGGTGGAAAGGTGTGGAATG | ||||
| IL10/B-TCGF | S: ACCAGATGGGCGACTTGTTG | 65 | 123 | NM_214041 |
| AS: TCTCTGCCTTCGGCATTACG | ||||
| IL12 p35 | S: GGCCTGCTTACCACTTGAAC | 64 | 180 | NM_213993 |
| AS: GCATTCATGGCCTGGAACTC | ||||
| IL12 p40 | S: CTGAAGAAGACGGCATCACG | 62 | 148 | NM_214013 |
| AS: AGGAGTGACTGGCTCAGAAC | ||||
| IL13 | A: TGGCGCTCTGGTTGACTCTG | 67 | 159 | NM_213803 |
| AS: CCATGCTGCCGTTGCATAGG | ||||
| IL15/IL-T | S: CTGAGGATGGCATTCATGTC | 60 | 164 | NM_214390 |
| AS: GGGATGAGCATCACTTTCAG | ||||
| IL17a/CTLA8 | A: CCAGACGGCCCTCAGATTAC | 66 | 103 | AB102693 |
| AS: CACTTGGCCTCCCAGATCAC | ||||
| IL18/IGIF | S: ACATCAAGCCGTGTTTGAGG | 60 | 129 | EU118362 |
| AS: CACTGCACAGAGATGGTTAC | ||||
| IL21 | S: GGCACAGTGGCCCATAAATC | 62 | 124 | NM_214415 |
| AS: GCAGCAATTCAGGGTCCAAG | ||||
| IL22/IL-TIF | S: AAGCAGGTCCTGAACTTCAC | 60 | 133 | AY937228 |
| AS: CACCCTTAATACGGCATTGG | ||||
| IL23 p19 | S: CTCCTTCTCCGCCTCAAGATCC | 70 | 82 | PEDE Blast |
| AS: TTGCTGCTCCATGGGCGAAGAC | 20050322S038657 | |||
| IL27 p28 | S: GCCCGCCACTTTGCTGAATC | 64 | 152 | EST BP439244 |
| AS: GGGCGAAGTGTCATGGAGAG | ||||
| IL33 | S: AGCTTCGCTCTGGCCTTATC | 63 | 126 | EST BX924734 |
| AS: GCTGACAGGCAGCAAGTACC | ||||
| IFN gamma | A: GCTCTGGGAAACTGAATGAC | 60 | 167 | NM_213948 |
| AS: TCTCTGGCCTTGGAACATAG | ||||
| iNOS | S: GAGAGGCAGAGGCTTGAGAC | 62 | 178 | EST BI344008 |
| AS: TGGAGGAGCTGATGGAGTAG | ||||
| LTA/ TNF beta | A: CTCCTCAGCGCTCAGAAGTC | 64 | 172 | NM_214453 |
| AS: GAGCGAAGGCTCCAAAGAAG | ||||
| MAdCAM-1 | A: AGCCTGGGCTCCGTAAAGTC | 68 | 155 | NM_001037998 |
| AS: TGGTCAGGGAAGGCGAACAC | ||||
| NK-lysin | S: ATGCGACGGAGAGCAGTTC | 60 | 156 | X85431 |
| AS: GTGTCCTCGTTGGGTTGTG | ||||
| NOD2 | A: GAGCGCATCCTCTTAACTTTC | 63 | 66 | NM_001105295 |
| AS: ACGCTCGTGATCCGTGAAC | ||||
| PBD-1 | S: ACCGCCTCCTCCTTGTATTC | 62 | 150 | NM_213838 |
| AS: CACAGGTGCCGATCTGTTTC | ||||
| PBD-2 | S: TTGCTGCTGCTGACTGTCTG | 62 | 180 | NM_214442 |
| AS: CTTGGCCTTGCCACTGTAAC | ||||
| PMAP-37/cathelicidin | S: GCAGTCCTCGGAAGCTAATC | 62 | 166 | L39641 |
| AS: CCCGTTCTCCTTGAAGTCAC | ||||
| PR-39/cathelicidin | S: TAATCTCTACCGCCTCCTGG | 62 | 151 | NM_214450 |
| AS: CCCGTTCTCCTTGAAGTCAC | ||||
| RPL-19 | S: AACTCCCGTCAGCAGATCC | 60 | 147 | AF435591 |
| AS: AGTACCCTTCCGCTTACCG | ||||
| RORC/ROR gamma | A: TTCAGTACGTGGTGGAGTTC | 60 | 141 | EST BP164723 |
| AS: TGTGGTTGTCAGCGTTGTAG | ||||
| Secretory | S: ACTGGTGTCGCTGGGAAGAG | 64 | 131 | EST CJ025705 |
| component | AS: GACCGTGAAGGTGCCATTGC | |||
| SipA | S: CCAACGCAATGGCGAGTCAC | 68 | 96 | NC_003197.1 |
| AS: GCCGTCTCCGTTTGATGCGT | ||||
| SMAD2 | A: TGAGTGCCTAAGTGACAGTG | 60 | 143 | EST DB812041 |
| AS: CCAGAAGAGCAGCAAATTCC | ||||
| SMAD3 | A: CGCAGAACGTCAACACCAAG | 62 | 139 | EST BX926114 |
| AS: AGCTCATGGTGGCTGTGAAG | ||||
| STAT3 | A: TGCAGCAGAAAGTGAGCTAC | 60 | 166 | NM_001044580 |
| AS: CCGGTCTTGATGACTAATGG | ||||
| STAT4 | A: ACCATTCGCTGACATCCTTC | 60 | 126 | AB20984 |
| AS: TGGGAGCTGTAGTGTTTACC | ||||
| STAT5 | A: CAGCCATCTGGAGGACTAC | 60 | 109 | EST CJ011824 |
| AS: CATCACGCCATCAAACCAC | ||||
| STAT6 | A: TCCCAGCTACGATCAAGATG | 60 | 171 | EST CN155407 |
| AS: AGTGAGAGTGTGGTGGATAC | ||||
| T-Bet | A: TCAATCCTACTGCCCACTAC | 60 | 151 | EST CJ014895 |
| AS: TTAGGAGACTCTGGGTGAAC | ||||
| TBP-1 | S: AACAGTTCAGTAGTTATGAGCCAGA | 60 | 153 | DQ845178 |
| AS: AGATGTTCTCAAACGCTTCG | ||||
| TGF beta | S: GAAGCGCATCGAGGCCATTC | 64 | 162 | NM_214015 |
| AS: GGCTCCGGTTCGACACTTTC | ||||
| TLR2 | A: ACGGACTGTGGTGCATGAAG | 62 | 101 | NM_213761 |
| AS: GGACACGAAAGCGTCATAGC | ||||
| TLR4 | A: TGTGCGTGTGAACACCAGAC | 62 | 136 | NM_001113039 |
| AS: AGGTGGCGTTCCTGAAACTC | ||||
| TLR5 | A: CCTTCCTGCTTCTTTGATGG | 61 | 124 | NM_001123202 |
| AS: CTGTGACCGTCCTGATGTAG | ||||
| TNF alpha/TNFSF2 | S: CCAATGGCAGAGTGGGTATG | 62 | 116 | X54859 |
| AS: TGAAGAGGACCTGGGAGTAG |