Figure 3.
Kinetics of Ab titres, plasma cell and memory B cell numbers after immunisation and boost-immunisation of calves with ovalbumin. Four calves were sub-cutaneously injected with 40 mg ovalbumin per animal in incomplete Freund's adjuvant (primary response, left panels), then boosted 49 days later with 10 mg ovalbumin per animal in incomplete Freund's adjuvant (secondary response, right panels). Blood samples were taken at various time-points following these injections and ovalbumin-specific IgG titres (Ab titre, A), plasma cell (B) and memory B cell (C) numbers were determined by ELISA and B cell ELISPOT. Results were expressed as ASC per 106 PBMC for plasma cells and induced-ASC per 106 cultured PBMC for memory B cells (mean of duplicate determinations).