Lymphocytes from genetically deficient mice in MyD88 are not able to produce acute IFN-γ after N. caninum infection. (A) Immunophenotyping of potential IFN-γ⁺ cells to the peritoneal cavity at 3, 7, and 10 days p.i. (n = 3 mice/group/date), determined by flow cytometry analysis of cell surface markers. Data are representative of two independent experiments. (B) Intracellular IFN-γ staining of immunophenotyped lymphocytes present in the peritoneal cavity of uninfected WT and MyD88^−/− and after 3 days p.i. The results show one representative animal of a total from three of each group/date. * Indicates significant statistical differences (mean ± SEM, p < 0.05).