Free access article

Vet. Res. 36 (2005) 629-643
DOI: 10.1051/vetres:2005023

Neutrophil apoptosis during experimentally induced Staphylococcus aureus mastitis

Zbysek Sladek^a, Dusan Rysanek^b, Helena Ryznarova^a and Martin Faldyna<sup>b

a</sup>  Department of Morphology, Physiology and Veterinary Sciences, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic
^b  Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic

(Received 8 October 2004; accepted 14 January 2005)

Abstract - The objective of this study was to determine whether neutrophil apoptosis and their consequent elimination by macrophages from the mammary gland is modulated by an infection caused by Staphylococcus aureus (S. aureus). The study was performed on twenty mammary glands of 5 virgin heifers. A buffered physiological solution (PBS) was administered as a means of control into the mammary glands of the heifers and after 168 h, the glands were inoculated with S. aureus. The samples of cell populations were obtained by lavages of the mammary glands in 4 intervals (24, 48, 72 and 168 h) after the experimental infection. Flow cytometry was used for determination of Annexin-V positivity and propidium iodide (PI) negativity of neutrophils. Light microscopy was used for determination of neutrophil karyopyknosis. Cytochemistry was used for the detection of myeloperoxidase-positive (MPO+) macrophages. Instillation of S. aureus resulted in an intramammary infection which persisted during the following experimental period. The total number of both Annexin-V-positive and PI negative neutrophils and karyopyknotic neutrophils peaked at 24 h after both of PBS and S. aureus administration. The highest percentages of Annexin-V-positive and PI negative neutrophils and karyopyknotic neutrophils were detected 48 and 168 h after PBS and S. aureus administration, respectively. The total number of MPO+ macrophages was the highest 24 h and 48 h after PBS and S. aureus administration, respectively; the percentage of MPO+ macrophages was the highest at 72 h in both cases. The dynamics of resolution of mastitis caused by S. aureus was very similar to the resolution of inflammatory response of the mammary gland after PBS administration. Mechanisms of cell pathogen elimination as well as inflammation resolution were very intensively involved; nevertheless, the mammary gland infection persisted. An early inclusion of the mechanisms of an acute inflammatory resolution thus paradoxically led to chronic infection.

Key words: Staphylococcus aureus / mastitis / neutrophil apoptosis

Corresponding author: Zbysek Sladek sladekz@seznam.cz

© INRA, EDP Sciences 2005

The OpenURL standard is a protocol for transmission of metadata describing the resource that you wish to access. An OpenURL link contains article metadata and directs it to the OpenURL server of your choice. The OpenURL server can provide access to the resource and also offer complementary services (specific search engine, export of references...). The OpenURL link can be generated by different means.

• If your librarian has set up your subscription with an OpenURL resolver, OpenURL links appear automatically on the abstract pages.
• You can define your own OpenURL resolver with your EDPS Account. In this case your choice will be given priority over that of your library.
• You can use an add-on for your browser (Firefox or I.E.) to display OpenURL links on a page (see http://www.openly.com/openurlref/). You should disable this module if you wish to use the OpenURL server that you or your library have defined.