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DOI: 10.1051/vetres:2000119
Vet. Res. 31 (2000) 241-246
Bovine respiratory syncytial virus:
first serological evidence in Uruguay
Mauro Costaa - Laura Garcíaa - Abdul S. Yunusb -
Daniel D. Rockemannb - Siba K. Samalb - Juan Cristinaa
aDepartamento de Técnicas Nucleares Aplicadas, Centro de Investigaciones Nucleares,
Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay
bVirginia-Maryland Regional College of Veterinary Medicine, University of Maryland
at College Park, Maryland 20742-3711, USA
(Reveived 22 June 1999; accepted 18 November 1999)
Abstract:
Bovine respiratory syncytial virus (BRSV) is a major cause of respiratory disease
in calves resulting in a substantial economic loss for the cattle industry worldwide. In
order to determine the presence of BRSV in Uruguay, an immunoenzymatic test was set up,
using a recombinant BRSV nucleocapsid (N) protein as the antigen. The N protein was produced
in sf9 insect cells by a recombinant baculovirus expressing the N protein. Serum samples
collected from one hundred cattle from four different geographic regions of Uruguay were
analyzed. Antibodies against the N protein of BRSV were detected in 95% of the serum samples
analyzed. These results show for the first time the presence of BRSV antibodies and suggest
a widespread BRSV infection in the cattle population of Uruguay.
Keywords:
respiratory syncytial virus / recombinant N protein / epidemiology / baculovirus / Uruguay
Résumé:
Résumé -
Première mise en évidence sérologique du virus syncytial respiratoire bovin en
Uruguay. Le virus syncytial respiratoire bovin (VSRB) est une cause majeure de maladie
respiratoire chez le veau, provoquant des pertes économiques importantes pour
l'industrie bovine dans le monde entier. Afin de mettre en évidence la présence du VSRB en
Uruguay, un test immunoenzymatique a été élaboré, qui utilise comme antigène une protéine
recombinante de nucléocapside (N) du VSRB. La protéine N a été produite dans des cellules
d'insecte sf9 par un baculovirus recombinant exprimant la protéine N. Des échantillons de
sérum prélevés sur100 bovins, provenant de quatre régions différentes d'Uruguay, ont été
analysés. Des anticorps dirigés contre la protéine N du VSRB ont été détectés dans 95% des
échantillons analysés. Ces résultats montrent, pour la première fois, la présence d'anticorps
anti-VSRB et suggèrent que l'infection par le VSRB est largement répandue dans la population
bovine en Uruguay.
Mots clé :
virus syncytial respiratoire bovin / protéine N recombinante / épidémiologie / baculovirus / Uruguay
Correspondence and reprints: Juan Cristina
Tel.: (598) 2 525 09 01; fax: (598) 2 525 08 95; cristina@cin1.cin.edu.uy
Copyright INRA, EDP Sciences
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