Free access
Issue
Vet. Res.
Volume 41, Number 1, January-February 2010
Number of page(s) 16
DOI http://dx.doi.org/10.1051/vetres/2009060
Published online 14 October 2009
How to cite this article Vet. Res. (2010) 41:12
How to cite this article: Vet. Res. (2010) 41:12
DOI: 10.1051/vetres/2009060

Time course differential gene expression in response to porcine circovirus type 2 subclinical infection

Anna Tomás 1, Lana T. Fernandes1, Armand Sánchez2 and Joaquim Segalés1, 3

1  Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra 08193, Barcelona, Spain
2  Dept. Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain
3  Dept. Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain

Received 16 February 2009; accepted 12 October 2009; published online 14 October 2009

Abstract - This study was aimed at characterizing the potential differences in gene expression in piglets inoculated with Porcine circovirus type 2 (PCV2), the essential causative agent of postweaning multisystemic wasting syndrome. Seven-day-old caesarean-derived, colostrum-deprived piglets were distributed into two groups: control (n = 8) and pigs inoculated with 105.2 TCID50 of the Burgos PCV2 isolate (n = 16). One control and three inoculated pigs were necropsied on days 1, 2, 5, and 8 post-infection (p.i.). The remaining pigs (four of each group) were sequentially bled on days 0, 7, 14, 21, and 29 p.i. (necropsy). Total RNA fromthe mediastinal lymph node (MLN) and lysed whole blood (LWB) samples were hybridized to Affymetrix Porcine GeneChip$^\circledR$. Forty-three probes were differentially expressed (DE) in MLN samples (FDR < 0.1, fold change > 2) and were distributed into three clusters: globally down-regulated genes, and up-regulated genes at early (first week p.i.) and late (day 29 p.i.) stages of infection. In LWB samples,maximal differences were observed at day 7 p.i., with 54 probes DE between control and inoculated pigs. Main Gene Ontology biological processes assigned to upregulated genes were related to the immune response. Six common genes were found in both types of samples, all of which belonged to the interferon signaling antiviral effector pathway. Down-regulated genes were mainly related to cell adhesion and migration in MLN, and cellular organization and biogenesis in LWB. Microarray results were validated by quantitative real-time PCR. This study provides, for the first time, the characterization of the early and late molecular events taking place in response to a subclinical PCV2 infection.


Key words: pig / time course / gene expression / porcine circovirus type 2 / immune response

Corresponding authors: anna.tomas@caubetcimera.es joaquim.segales@cresa.uab.cat

© INRA, EDP Sciences 2009