Expression of three intelectins in sheep and response to a Th2 environmentAnne T. French1, Pamela A. Knight1, W. David Smith2, Judith A. Pate1, Hugh R.P. Miller1 and Alan D. Pemberton1
1 Royal (Dick) School of Veterinary Studies, Division of Veterinary Clinical Sciences University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian, EH25 9RG, United Kingdom
2 Moredun Research Institute, Pentlands Science Park, Penicuik, Midlothian, EH26 0PZ, United Kingdom
Received 23 March 2009; accepted 22 June 2009; published online 24 June 2009
Abstract - Sheep intelectin1 and sheep intelectin3 (sITLN1 and sITLN3) were cloned and sequenced. The amino acid sequences of sITLN1 and sITLN3 shared 86% and 91% homology with the previously cloned sheep intelectin2 (sITLN2), respectively. Expression of sITLN1 and sITLN3 transcript was demonstrated in abomasum, lung, colon and gastric lymph node, terminal rectum, skin, jejunum, mesenteric lymph node, ileal peyer's patches, brain, kidney, liver, spleen, skin, ear pinna, heart and ovary in normal sheep tissues. sITLN2 transcript expression was restricted to the abomasal mucosa in normal sheep tissues. Using a non selective chicken anti-intelectin antibody, tissue intelectin protein was demonstrated in mucus neck cells in the abomasum, mucus cells in the colon, free mucus in ileum, goblet cells in the lung, small intestinal epithelium and brush border, epidermal layer of the skin and skin sebaceous glands. The expression of the three sITLN transcripts was examined in two nematode infections in sheep known to induce a Th2 response; a Teladorsagia circumcincta challenge infection model and a Dictyocaulus filaria natural infection. The three sITLN were absent in unchallenged naïve lambs and present in the abomasal mucosa of both naïve and immune lambs following T. circumcincta challenge infection. Upregulation of sITLN2 and sITLN3 was shown in sheep lung following D. filaria natural infection. Intelectins may play an important role in the mucosal response to nematode infections in ruminants.
Key words: sheep intelectin / sequence / tissue distribution / parasite response
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© INRA, EDP Sciences 2009