Open Access
Issue
Vet. Res.
Volume 38, Number 6, November-December 2007
Page(s) 871 - 882
DOI http://dx.doi.org/10.1051/vetres:2007035
Published online 20 September 2007
How to cite this article Vet. Res. (2007) 871-882
Vet. Res. 38 (2007) 871-882
DOI: 10.1051/vetres:2007035

Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

Yaohong Zhua, b, Caroline Fossumc, Mikael Bergd and Ulf Magnussona, b

a  Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, PO Box 7054, 75007 Uppsala, Sweden
b  Centre for Reproductive Biology in Uppsala, Uppsala, Sweden
c  Division of Immunology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, 75123 Uppsala, Sweden
d  Division of Parasitology and Virology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, 75007 Uppsala, Sweden

(Received 13 February 2007; accepted 11 June 2007; published online 20 September 2007)

Abstract - Twelve healthy primiparous sows received intramammary inoculation with Escherichia coli (serotype O127) during the 24-h period preceding parturition. Mammary gland biopsy samples were taken immediately before inoculation (0 h) and from the inoculated and the contralateral non-inoculated glands 24 h after inoculation. The analyses of interleukin-1 beta (IL-1$\beta$), IL-6, IL-8, and tumor necrosis factor alpha (TNF-$\alpha$) by immunohistochemistry revealed that the production of these proinflammatory cytokines significantly increased in the inoculated mammary glands of sows that developed clinical signs of mastitis (affected group, n=4) 24 h after inoculation. This was also true for IL-8 in the inoculated mammary glands of sows that did not develop clinical signs of mastitis (nonaffected group, n=8). Sows that developed clinical signs of mastitis displayed significantly lower constitutive production of IL-1$\beta$ than did sows that remained clinically healthy. The data indicate that the development of clinical symptoms of coliform mastitis in the sow is associated with a locally increased proinflammatory cytokine production in response to intramammary E. coli infection.


Key words: cytokine / immunohistochemistry / mastitis / pig / E. coli

Corresponding author: ulf.magnusson@kv.slu.se

© INRA, EDP Sciences 2007