Free access
Issue
Vet. Res.
Volume 38, Number 6, November-December 2007
Page(s) 795 - 808
DOI http://dx.doi.org/10.1051/vetres:2007032
Published online 31 August 2007
How to cite this article Vet. Res. (2007) 795-808
Vet. Res. 38 (2007) 795-808
DOI: 10.1051/vetres:2007032

Effects of CpG-B ODN on the protein expression profile of swine PBMC

Govindarajulu Nagarajana, Cheng-Chin Kuo*a, Chi-Ming Liangb, c, d, Chi-Min Chene and Shu-Mei Lianga, b

a  Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Sec. 2, 11529 Taipei, Taiwan
b  Genomics Research Center, Academia Sinica, No. 128, Academia Road, Sec. 2, 11529 Taipei, Taiwan
c  Institute of Biological Chemistry, Academia Sinica, No. 128, Academia Road, Sec. 2, 11529 Taipei, Taiwan
d  Division of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli, Taiwan
e  Division of Animal Medicine, Animal Technology Institute Taiwan, Miaoli, Taiwan

(Received 15 September 2006; accepted 26 April 2007; published online 31 August 2007)

Abstract - The CpG motif within bacterial DNA is a potent immuno-stimulatory moiety. Here, using a 2-D electrophoretic approach, we investigated the effect of synthetic oligodeoxynucleotide containing a B type CpG motif (CpG-B ODN) on the protein expression profile of swine peripheral blood mononuclear cells (PBMC). We found that several proteins including spondin 1, N-acetolactate alpha linked acidic dipeptidase; V kappa light chain, T cell receptor variable alpha chain, heat shock protein (Hsp) 60, Hsp70, KIAA0857 protein, and PNAS-146 were up-regulated in PBMC by CpG-B ODN stimulation. Further studies showed that CpG-B ODN-mediated Hsp60, Hsp70 and Hsp90 expressions were closely associated with the TLR9 signalling pathway. Pretreatment with inhibitors of Hsp70, Hsp90 and TLR9 all blocked the CpG-B ODN-mediated anti-apoptotic effect in swine PBMC. These results suggest that CpG-B ODN treatment of swine PBMC may enhance the expression of biologically active proteins, notably spondin 1, V kappa light chain, T cell receptor variable alpha chain and Hsps, which may play an important role in CpG-B ODN-mediated activation of immune responses and enhancement of swine PBMC survival.


Key words: heat shock proteins / CpG ODN / TLR9 / proteomics / apoptosis

Corresponding author: smyang@gate.sinica.edu.tw

© INRA, EDP Sciences 2007