Ketamine inhibits LPS-induced tumour necrosis factor-alpha and interleukin-6 in an equine macrophage cell lineDaniëlle P.K. Lankvelda, Sarah Bullb, Paul Van Dijka, Johanna Fink-Gremmelsb and Ludo J. Hellebrekersa
a Section Anaesthesiology and Intensive Care, Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 12, 3584 CM, Utrecht, The Netherlands
b Department of Veterinary Pharmacology Pharmacy and Toxicology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 16, 3584 CM, Utrecht, The Netherlands
(Received 24 May 2004; accepted 5 October 2004)
Abstract - Ketamine is widely used in equine anaesthesia. Beside its anaesthetic and analgesic properties, ketamine possesses a cytokine-modulating activity. However, to date, no data are available regarding the inhibitory effect of ketamine on the cytokine response in horses. In horses, cytokines such as tumour necrosis factor-alpha (TNF- ) and interleukin-6 (IL-6) play a pivotal role in the pathogenesis of equine endotoxaemia following gastrointestinal disorders. Hence, the objective of this study was to assess the influence of ketamine on LPS-induced TNF- and IL-6 formation in an equine macrophage cell line (eCAS cells). The results demonstrate a cytokine-modulating activity of ketamine in an equine cell line, suggesting a beneficial role for ketamine in the treatment of equine endotoxaemia.
Key words: ketamine / cytokine / equine endotoxaemia / eCAS
Corresponding author: Daniëlle P.K. Lankveld D.P.K.Lankveld@vet.uu.nl
© INRA, EDP Sciences 2005