Free Access
Issue
Vet. Res.
Volume 34, Number 4, July-August 2003
Page(s) 423 - 433
DOI https://doi.org/10.1051/vetres:2003017
How to cite this article Vet. Res. (2003) 423-433
Vet. Res. 34 (2003) 423-433
DOI: 10.1051/vetres:2003017

Experimental Coxiella burnetii infection in pregnant goats: excretion routes

Nathalie Arricau Bouvery, Armel Souriau, Patrick Lechopier and Annie Rodolakis

Pathologie Infectieuse et Immunologie, INRA, Tours-Nouzilly, 37380 Nouzilly, France
(Received 15 October 2002, accepted 21 March 2003)

Abstract
Q fever is a widespread zoonosis caused by Coxiella burnetii. Infected animals, shedding bacteria by different routes, constitute contamination sources for humans and the environment. To study Coxiella excretion, pregnant goats were inoculated by the subcutaneous route in a site localized just in front of the shoulder at 90 days of gestation with 3 doses of bacteria (10 8, 10 6 or 10 4 I.D.). All the goats aborted whatever the dose used. Coxiella were found by PCR and immunofluorescence tests in all placentas and in several organs of at least one fetus per goat. At abortion, all the goats excreted bacteria in vaginal discharges up to 14 days and in milk samples up to 52 days. A few goats excreted Coxiella in their feces before abortion, and all goats, excreted bacteria in their feces after abortion. Antibody titers against Coxiella increased from 21 days post inoculation to the end of the experiment. For a Q fever diagnostic, detection by PCR and immunofluorescence tests of Coxiella in parturition products and vaginal secretions at abortion should be preferred to serological tests.


Key words: Coxiella burnetii / goat / experimental infection / abortion / shedding

Correspondence and reprints: Nathalie Arricau Bouvery Tel.: (33) 2 47 42 76 34; fax: (33) 2 47 42 77 79;
    e-mail: Nathalie.Bouvery@tours.inra.fr

© INRA, EDP Sciences 2003

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.